Autofluorescence is your new best friend!

Photothermal Spectroscopy Corp (PSC) is pleased to announce the launch of its NEW Widefield O-PTIR Chemical Imaging system.

Widefield O-PTIR™ is a new sub-micron IR, high-speed widefield IR chemical imaging mode, with simultaneous fluorescence imaging, for the measurement of a wide range of labelled and autofluorescent biological samples.

It is powered by a patented technique – Fluorescence Detection Photothermal Infrared (FL-PTIR).

Please join Dr Craig Prater, CTO of PSC and Widefield O-PTIR co-inventor, to learn more about Widefield O-PTIR Chemical Imaging and the base technology of O-PTIR.

Webinar Highlights

• Introduction to the new breakthrough technique of sub-500nm Widefield O-PTIR and simultaneous fluorescence imaging with various life science applications, from tissues, plants and microorganisms
• Learn how IR chemical images can be collected in seconds, with full hyperspectral images in minutes with 130nm pixel resolution across 262,144 (512×512) pixels
• Overview of submicron O-PTIR and simultaneous Raman with co-located fluorescence imaging
• Comparisons and advantages over current traditional FTIR and Raman microscopy techniques
• A review of applications, drawing on a variety of recent high impact publication examples

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

Sub-micron IR characterisation of fluid inclusions within minerals:

Molecular fractionation of ancient organic compounds in deeply buried halites

Additionally, Dr Mustafa Kansiz, Director of Product Management at Photothermal Spectroscopy Corp, will provide a brief technique overview, covering submicron simultaneous IR+Raman with co-located fluorescence widefield imaging, with advances now delivering sub-500nm IR spatial resolution.

Learn how;

• O-PTIR achieves the same high spatial resolution (sub-micron) of Raman microscopy, but with the rich information content of IR spectroscopy with high sensitivity in seconds
• O-PTIR eliminates many traditional challenges of Raman microscopy, such poor sensitivity of cells and fluorescence interferences
• Co-located widefield epi fluorescence for guided measurements without any sample registration needed
• O-PTIR enables exploration of the synergistic complementarity between IR and Raman while allowing the user to collect submicron IR+Raman spectra simultaneously, from the same spot, at the same time with the same spatial resolution

These breakthrough advances will be presented in this two-part event, together with a range of application examples, from life sciences, to particulates/microplastics, failure analysis and materials sciences.

This webinar will be recorded for later on-demand viewing, with opportunities to ask questions during and after the webinar.

Part 1: Introduction to O-PTIR

• Overview of current direct IR (FTIR & QCL) and Raman microspectroscopic techniques applied to MPs
• Recognizing and understanding spectral artefacts/inconsistencies and how they significantly reduce MP ID accuracy
• Introduction to featurefindIR™ software automation and reporting for Submicron O-PTIR and Simultaneous Raman microscopy
• How cross-polarized visible light microscopy can be used to enhance particle detection
• Combined fluorescence (Nile Red) imaging for polymer particle locating (amongst inorganics), followed by O-PTIR analysis for increased efficiency and time savings
• See examples applied to MPs and microfibers from <500nm to mm’s and how the spectra are reliable and reproducible, regardless of particle shape and size

Part 2: Live demo

• See, in real-time, how easy it is to use featurefindIR™ for MP analysis in seconds, ranging from <500nm to mm’s and immediately search spectral databases and generate reports with key particle shape/size information together with chemical identification.
• The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

Simultaneous SERS/SEIRA with single molecule detection – A world first

A summary of the webinar includes;

• Concurrent, simultaneous acquisition of SEIRA and SERS from the same plasmonic hotspot
• Acquisition of simultaneous infrared and Raman spectra from a single molecule or molecular cluster
• Demonstration of a dual enhancement for SEIRA, wherein the photothermal optical probe and the infrared absorption are both enhanced, enabling very sensitive, photothermally detected SEIRA, down to the single molecule
• Application of dual SEIRA and SERS to interrogate material collected from a surface onto an atomic force microscope tip

This work also serves to demonstrate the utility and sensitivity of the O-PTIR+Raman system, with the unique combination of SEIRA and SERS providing very high sensitivity and complementary information.
Additionally, Dr Mustafa Kansiz, Director of Product Management and Marketing at Photothermal Spectroscopy Corp, will provide a brief technique overview, comparisons to traditional IR and Raman spectroscopy, covering submicron simultaneous IR+Raman together with a range of application examples in this two part event.

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

New sub-500nm IR with simultaneous Raman and co-located fluorescence microscopy: applications and live demo

Part 1: Overview

• Overview of current direct IR (FTIR & QCL) and Raman micro spectroscopic techniques and fluorescence microscopy
• Introduction to Submicron O-PTIR, simultaneous Raman microscopy and the benefits of co-located fluorescence
• Examples covering neuroscience (protein aggregation and disorder), cells, plants, microplastics and industrial failure analysis

Part 2: Live Demonstration:

• See, in real-time, how easy it is to collect sub-500nm IR and simultaneous Raman data with co-located fluorescence in seconds from a range of sample types

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

Part 1: Overview

• Overview of current direct IR (FTIR & QCL) and Raman micro spectroscopic techniques and fluorescence microscopy
• Introduction to Submicron O-PTIR, simultaneous Raman microscopy and the benefits of co-located fluorescence
• Examples covering neuroscience (protein aggregation and disorder), cells, plants, microplastics and industrial failure analysis

Part 2: Live Demonstration:

• See, in real-time, how easy it is to collect sub-500nm IR and simultaneous Raman data with co-located fluorescence in seconds from a range of sample types

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

Submicron simultaneous IR+Raman – Breakthrough research from life sciences to cultural heritage to polymers

How O-PTIR is being used at multi-user facilities to enable breakthrough research

A summary of the webinar includes;

• Introduction to submicron O-PTIR and simultaneous Raman (IR+Raman) microscopy with application examples
• Submicron IR (O-PTIR) applied to;
  – Cultural heritage – View publication here
  – Neuroscience – View publication here
  – Kidney stones
  – Hair analysis
  – Polymer orientation

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

Submicron simultaneous IR+Raman – Breakthrough research from life sciences to cultural heritage to polymers

How O-PTIR is being used at multi-user facilities to enable breakthrough research

Dr. Mustafa Kansiz
Director of Product Management and Marketing
Photothermal Spectroscopy Corp

A summary of the webinar includes;

• Introduction to submicron O-PTIR and simultaneous Raman (IR+Raman) microscopy with application examples
• Submicron IR (O-PTIR) applied to;
  – Cultural heritage – publication here
  – Neuroscience – publication here
  – Kidney stones
  – Hair analysis
  – Polymer orientation

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.

• Single cell bacteria/spores
• Single eukaryotic cells and tissues
• Polymer phase dispersion samples
• Particulates (microplastics)

O-PTIR breaks all the known limitations and issues with conventional FTIR/QCL microscopy with a summary of capabilities listed below;

• Ability to perform simultaneous, submicron IR+Raman microscopy (same spot, same time, same resolution)
• Submicron (~500nm) IR or (IR+Raman) spatial resolution
• No IR dispersive scatter artefacts (Mie scatter)
• Easy-to-use reflection mode (non-contact), delivering high quality spectra that are fully comparable to FTIR transmission data
• Single frequency (wavenumber) imaging, no more having to collect slow hyperspectrals
• Little to no sample prep, measure from 100nm to >10mm in thickness, rough or smooth.
• Zero fluorescence interference for the IR, regardless of laser wavelength or sample
• Excellent spectral sensitivity in ~1sec
• No laser sample damage or phototoxicity (laser powers <100mW)

Dr Mustafa Kansiz, Director of Product Management and Marketing at PSC, will present an overview of the technique, with examples of applications from life sciences, polymer research, particulates (microplastics), industrial failure analysis and more, before switching to a live online instrument demo.

The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar

This new approach dramatically improves the identification accuracy of results compared to single technique measurement and database searching.

Available for viewing

Presenters:
Dr. Curtis Marcott, Senior Partner, Light Light Solutions
Dr. Craig Prater, CTO, Photothermal Spectroscopy Corp

• Sub-micron spatial resolution
• Transmission FTIR quality spectra in reflection mode on thick samples
• Non-contact optical technique with none of the limitations of ATR
• Spatial resolution and sample preparation advantages found in Raman technology, with IR sensitivity
• Works on any sample prepared for IR or Raman spectroscopy