The advancement of analytical methods and systems are paving the way for innovation in academic, industrial and government sectors. As a result of the fast-paced progress, more research questions are asked that surpass the limits of these technologies. The mIRage IR Microscope is now answering these questions using Optical Photothermal IR (O-PTIR) spectroscopy, as well as IR+Raman – simultaneous submicron IR and Raman microscopy. With more chemical characterization requirements being met, mIRage is ushering in the next generation of applications and technology developments in numerous industries.
Submicron line scan of polystyrene beads embedded in epoxy
Film defect identification
Polymers are present in virtually all products we interact with daily. With increasing environmental awareness, polymer science is looking at more novel and complex solutions to improve functionality and reduce environmental impact. These requirements often exceed the limits of traditional IR microscopy, especially when it comes to spatial resolution. The mIRage IR Microscope, with its unique submicron spatial resolution using a non-contact reflection mode technique, is able to meet even the most demanding of analytical and sample characterization needs.
Application note: Submicron resolution IR spectroscopy and imaging of multilayer films for food packaging
Imaging and spectroscopy of bioplastic laminates
O-PTIR Scan of Bioplastic Laminate
Linear sampling scan spanning 8.0µm measured every 100µm apart (plotted only every 200 nm and across 2 mm for clarity) across the boundary of the bioplastic laminate, moving from the pure PHBHx layer to the pure PLA layer.
Gradual spectral changes over the space much greater than the optical resolution suggest the mixed distribution of PLA and PHBHx without any sharp boundary.
No clear isosbestic point indicates that the system is not a simple binary mixture.
PLA and PHBHx contributions are overlapped and mingled in the fingerprint region
Little to no sample preparation of a multilayer film
Submicron spatial resolution between film layers
Submicron O-PTIR imaging of live cells in water
were collected using a 0.5 µm step size in transmission mode.
IR+Raman analysis of red blood cells
IR imaging of mineral: protein distribution in mouse bone
Left: Hyperspectral array images of mouse bone taken at 1047 cm-1 (second from left) and 1660 cm-1 (second from right) show mineral and protein distribution, respectively. Right: Corresponding spectra taken from the inner bone, showing a higher absorption for phosphate.
Data courtesy of Prof. Nancy Pleshko, Dr. Mugdha Padalkar, and Jessica M. Falcon, Temple University
Pharmaceutical analysis often requires very precise and accurate measurements. With often tightening regulatory standards, development of new analytical methods must correlate. With unique submicron spatial chemical resolution, combined with simultaneous IR and Raman microscopy (IR+Raman), the mIRage IR Microscope is uniquely placed to meet and exceed the analytical needs of the pharmaceutical industry, across applications areas such as failure analysis, subvisibles and API/Excipient distribution in solid dose or emulsion-based formulations.