Please join Dr Yeran Bai, Dept of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, and Dr Mustafa Kansiz from PSC, in this two-part webinar to learn how submicron IR (O-PTIR) spectroscopy and imaging is being applied for the study of lipid metabolic heterogeneity in single cells, using novel IR tags, based on azide conjugated fatty acid precursors.
Traditional single-cell metabolic analysis faces significant challenges—bulky fluorescent labels interfere with cellular processes, lack specificity for metabolic products, and cannot provide direct chemical information about newly synthesized biomolecules. Conventional vibrational imaging techniques are limited by spatial resolution and sensitivity for detecting subtle metabolic changes in living cells.
Optical Photothermal Infrared Spectroscopy (O-PTIR) is revolutionizing single-cell metabolic analysis.
This breakthrough technique enables label-free, high-resolution detection of lipid metabolism using biorthogonal vibrational probes that provide selective detection without interference from cellular endogenous molecules. O-PTIR delivers sub-500nm IR spatial resolution with simultaneous fluorescence imaging for comprehensive single-cell metabolic characterization in both 2D and 3D culture systems.
Join us to discover how O-PTIR is transforming cellular metabolism research—finally making it possible to directly image newly synthesized lipids with unprecedented specificity and spatial resolution at the single-cell level.
Webinar Highlights: ✅ Breaking the Single-Cell Barrier – Direct imaging of lipid metabolism with sub-500nm IR spatial resolution in living cells
✅ Biorthogonal IR Probes – Novel azide conjugated fatty acid precursors for selective metabolic detection without cellular interference
✅ O-PTIR vs. Traditional Methods – Advantages over fluorescence imaging and conventional vibrational spectroscopy for metabolic analysis
✅ Simultaneous Multi-Modal Imaging – Combined IR spectroscopy with co-located fluorescence for cell-type identification
✅ 2D and 3D Culture Systems – Applications across various human-derived culture models
✅ Real-World Applications – Case studies demonstrating lipid metabolic heterogeneity analysis in single cells
✅ Broader Applications – Examples spanning life sciences, microplastics, failure analysis, and materials sciences
Who Should Attend? 🔬 Cell Biology Researchers – Scientists studying cellular metabolism, lipid biology, and single-cell heterogeneity
🏥 Biomedical Scientists – Researchers investigating metabolic diseases, cancer metabolism, and therapeutic targets
🧪 Analytical Chemists – Professionals developing new methods for cellular and molecular analysis
🌍 Life Sciences Industry – Experts in drug discovery, biotechnology, and pharmaceutical development
⚡ Materials & Environmental Scientists – Researchers applying advanced spectroscopy across multiple disciplines
Don’t miss this opportunity to gain cutting-edge insights into single-cell metabolic imaging!